貂源伪狂犬病病毒的分离鉴定及gE基因分子特征

doi:10.11843/j.issn.0366-6964.2015.07.026

Abstract

Abstract:

An epidemic disease characterized by neurological symptoms，diarrhea and acute death phenomenon in different ages of mink occurred in many fur farms of China since 2014.One pseudorabies virus (PRV) was identified by cell culture，plaque purification，morphology observation and animal inoculation experiment，named Mink 1.The isolate was able to cause typical CPE in MDCK cells，and cause itch，death in experimentally infected rabbit.Virus particles were round，with envelope，diameter of about 150 nm under the electron microscope.The gE of the Mink 1 was amplified by PCR and the results showed that PRV were found in detected animal.Sequence alignments indicated that the gE genes from the mink shared 99.4% identity with swine PRV isolated in 2012，located in a relatively independent branch.PRVs isolated had the same sequence features with two consecutive bases insertions of GAC and ACG at sites of 142-144 and 1 487-1 490 in gE compared with the classical strains.The above experimental results confirmed that the case is caused by PRV infection.

CLC Number:

S852.659.1

RUI Ping，LIU Yao-zong，MA Zeng-jun，WANG Qiu-yue，YANG Cai-ran，LIU Xie-rong. Identification and Glycoprotein E Characteristic of Pseudorabies Virus from Mink[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1268-1272.

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Identification and Glycoprotein E Characteristic of Pseudorabies Virus from Mink

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